Algal Culturing TechniquesRobert A. Andersen Academic Press, 2005. 1. 21. - 578ÆäÀÌÁö Algal Culturing Techniques is a comprehensive reference on all aspects of the isolation and cultivation of marine and freshwater algae, including seaweeds. It is divided into seven parts that cover history, media preparation, isolation and purification techniques, mass culturing techniques, cell counting and growth measurement techniques, and reviews on topics and applications of algal culture techniques for environmental investigations. Algal Culturing Techniques was developed to serve as both a new textbook and key reference for phycologists and others studying aquatic systems, aquaculture and environmental sciences. Students of algal ecology, marine botany, marine phycology, and microbial ecology will enjoy the hands-on methodology for culturing a variety of algae from fresh and marine waters. Researchers in industry, such as aquaculture, pharmaceutical, foodstuffs, and biotechnology companies will find an authoritative and comprehensive reference.
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1 Historical Review of Algal Culturing Techniques | 1 |
2 Freshwater Culture Media | 13 |
3 Marine Culture Media | 21 |
4 Trace Metal Ion Buffers and Their Use in Culture Studies | 35 |
5 Sterilization and Sterile Technique | 65 |
6 Traditional Microalgae Isolation Techniques | 83 |
7 Automated Isolation Techniques for Microalgae | 101 |
8 Purification Methods for Microalgae | 117 |
16 Counting Cells in Cultures with the Light Microscope | 239 |
17 Phytoplankton Cell Counting by Flow Cytometry | 253 |
18 Measuring Growth Rates in Microalgal Cultures | 269 |
19 Using Cultures to Investigate the Physiological Ecology of Microalgae | 287 |
20 Analysis of Algal Pigments by HighPerformance Liquid Chromatography | 327 |
21 Endogenous Rhythms and Daylength Effects in Macroalgal Development | 347 |
22 Viral Contamination of Algal Cultures | 365 |
23 Control of Sexual Reproduction in Algae in Culture | 389 |
9 Isolation and Purification Techniques for Macroalgae | 133 |
10 Perpetual Maintenance of Actively Metabolizing Microalgal Cultures | 145 |
11 LongTerm Macroalgal Culture Maintenance | 157 |
12 Cryopreservation Methods for Maintaining Microalgal Cultures | 165 |
The Light and Dark Sides of Growing Algae | 189 |
14 Culturing Microalgae in Outdoor Ponds | 205 |
15 Mariculture of Seaweeds | 219 |
Encystment and Excystment | 399 |
Ex Situ Conservation of Threatened Algal Species | 419 |
Appendix A Recipes for Freshwater and Seawater Media | 429 |
Glossary | 539 |
Name Index | 545 |
565 | |