GB 4789.44-2020: Translated English of Chinese Standard. (GB 4789.44-2020, GB4789.44-2020): National Food Safety Standard - Food microbiological examination - Vibrio vulnificus
https://www.chinesestandard.net, 2021. 3. 20. - 23페이지
This Standard specifies the examination method for Vibrio vulnificus in aquatic products. This Standard applies to the examination of Vibrio vulnificus in aquatic products such as fish, shrimp, crab, shellfish.
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Results and reports
Appendix A Media and reagents
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15.0 g Distilled 5.0 g Sodium according adjust the pH agar medium agar plate amount amplified bands apparatus autoclave at 121 biochemical characteristics blank control bp appears buffer Cellobiose CENTRIFUGE colony completely dissolved Composition Composition Peptone culture deionized water detection determined distilled water DNA template electrophoresis ethanol expected size 519 extraction g Beef powder g Distilled gene Glucose Gram staining Halophilic test homogenate hydrochloric acid solution identification iron agar slant later means mol/L hydrochloric acid mol/L sodium hydroxide Negative control ONPG oxidase test PCR reaction PCR testing phosphate PICK PNCC enrichment broth positive Preparation method DISSOLVE quality control system r/min reagent result result is determined sample sodium chloride trisaccharide sodium chloride tryptone sodium hydroxide solution solution to adjust staining solution standard strain Sterile strain of Vibrio supernatant system is normal Table test tubes trisaccharide iron agar tryptone soy agar Vibrio vulnificus