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The following show the circular of information, data card, and forms for sending reports on examination of sputum for tubercle bacilli contained in each outfit. The outfit consists of a double mailing case containing a bottle with 5 per cent carbolic acid:

West Virginia State Board of Health, Hygienic Laboratories,

Morgantown, W. Va.
Bacteriological Department.

DIAGNOSIS OF TUBERCULOSIS.

Directions for Collecting Sputum-Outfits for the collection of sputum including bottles containing five per cent carbolic acid, and blanks for particulars of cases with copies of these directions, will be found at the regular outfit stations.

The expectoration discharged in the morning is to be preferred. If the expectoration be scanty, the entire amount discharged in twentyfour hours should be collected. Care should be taken that the contents of the stomach, particles of food, etc., are not discharged during the act of expectoration and collected instead of pulmonary sputum. Purulent, cheesy, and muco-purulent sputa most frequently contain the bacilli; pure mucus, blood or saliva do not as a rule contain the bacilli. When hemorrhage has occurred, some purulent, cheesy muco-purulent sputum should, if possible, be collected for the examina

tion.

Collect sputum in a perfectly clean receptacle, then transfer to bottle furnished by the laboratory. Do not pour out carbolic acid and do not fill the bottle over two-thirds. Put cork in tightly so as to avoid leakage en route. Enclose the bottle in the laboratory package, together with the blank properly filled out, and forward at once by mail to the Bacteriologist, State Hygienic Laboratory, Morgantown, W. Va. Postage (12 cents) must be prepaid. A report will not be made unless the blank is properly filled out. Address all communications concerning specimens to the Bacteriologist, State Hygienic Laboratory, Morgantown, W. Va.

DO NOT POUR OUT THE CARBOLIC ACID.

A. ARKIN, Ph. D., M. D.,
Chief Bacteriologist.

West Virginia State Board of Health.
Hygienic Laboratories.

Morgantown, W. Va.

(This blank must be filled out in full and returned with specimen.)

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Number of Specimens submitted from this patient. First, second, Third,

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Shall report be sent at your expense by telegraph?..

FOR LABORATORY RECORD

(Do not fill out)

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Hour..

West Virginia State Board of Health
Hygienic Laboratories.
Morgantown, W. Va.

RULES GOVERNING THE EXAMINATION OF SPUTUM.

The examination of tubercular sputum even under the most favorable conditions is attended with some danger. To protect those making the examination in this laboratory the following rules are absolutely necessary and will be enforced:

1. Sputum must be submitted in the outfits supplied by the laboratory for that purpose. Indiscriminate containers must not be used.

2. Sputum will not be examined if leakage from the bottle has occurred or if any sputum is on outside of bottle.

Failure to observe these rules will result in specimens being refused examination.

Dr.

Dear Sir:

West Virginia State Board of Health
Hygienic Laboratories.
Morgantown, W. Va.

BACTERIOLOGICAL DEPARTMENT.

The specimen of sputum submitted by you from

on

shows the presence of the bacilli of tuberculosis.

Very truly yours,

A. ARKIN, Ph. D., M. D., Chief Bacteriologist.

Laboratory outfits MUST be used-otherwise specimens will not be examined.

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does not show the presence of the bacilli of tuberculosis.

Very truly yours,

A. ARKIN, Ph. D., M. D., Chief Bacteriologist.

NOTE-A single negative result is NOT conclusive proof that the patient does not have tuberculosis. Other specimens will be examined if desired.

Laboratory outfits MUST be used-Otherwise specimens will not be

The following represent information blank, data card, report blanks used in Diphtheria.

West Virginia State Board of Health

Hygienic Laboratories.
Morgantown, W. Va.

BACTERIOLOGICAL DEPARTMENT.

DIPHTHERIA DIAGNOSIS.

Directions.

1. Do not use and antiseptics for at least five hours previous to making the swab.

2. Do not allow the swab to come in contact with anything except the infected throat.

3. Rub the swab thoroughly over the suspected area as far back on the pharynx or nares as possible according to the location of the membrane.

4.

Examinations will not be made unless cards are properly filled out and swabs submitted to us in containers supplied from this laboratory. (Postage required prepaid, 6 cents.)

5. No case of diphtheria should be released from quarantine until two regular consecutive cultures taken at intervals of two days are reported negative.

6. Diphtheria outfits are to be obtained from distributing stations in your immediate vicinity. In case no station is near, outfits can be obtained upon request from the Hygienic Laboratories.

A. Arkin, Ph. D., M. D.,

Chief Bacteriologist.

DATA CARD FOR DIPHTHERIA.

Hygienic Laboratories-West Virginia State Board of Health.

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(Questions between heavy lines must be answered in full by physician)

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REPORT BLANKS FOR DIPHTHERIA.

West Virginia State Board of Health
Hygienic Laboratories.
Morgantown, W. Va.

BACTERIOLOGICAL DEPARTMENT.

Dr.

Dear Sir:

The culture submitted by

does not show the presence of the bacilli of diphtheria.

Very truly yours,

A. ARKIN, Ph. D., M. D., Chief Bacteriologist. NOTE- -A single negative result should not be considered conclusive, especially if the case looks suspicious.

Laboratory outfits MUST be used-otherwise specimens will not be examined.

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A. ARKIN, Ph. D., M. D., Chief Bacteriologist. Laboratory outfits MUST be used-otherwise specimens will not be examined.

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does not show the presence of typical diphtheria bacilli but does show a suspicious organism. Please submit another culture.

Very truly yours,

A. ARKIN, Ph. D., M. D., Chief Bacteriologist. Laboratory outfits MUST be used-otherwise specimens will not be

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