directly with infectious material which has been subjected to the action of a disinfectant, have been made by numerous observers. The proof of disinfection in this case is failure to produce the characteristic symptoms which result from inoculation with similar material not disinfected. Thus Duvaine found that the blood of an animal just dead from the disease known by English writers as anthrax or splenic fever (Fr., charbon), inoculated into a healthy rabbit or guinea pig, in the smallest quantity, infallibly produces death in two or three days; and the blood of these animals will again infect and cause the death of others, and so on indefinitely. This anthrax blood was therefore infectious material, which could be utilized for experiments relating to the comparative value of disinfectants. Duvaine made many such experiments. not only with the blood of anthrax, but also with that of a fatal form of septicemia in rabbits, which is known by his name. Other investigations have followed up these experiments upon infectious material of the same kind, and also upon material from other sources, e. g, the infectious material of glanders, of tuberculosis, of symptomatic anthrax, of fowl cholera, of swine plague, etc.

It has been proved that the infectious agent in all of the diseases mentioned is a living germ, and that disinfection consists in destroying the vitality of this germ. But in experiments made with blood or other material obtained directly from diseased animals, the results would be just as definite and satisfactory if we were still ignorant as to the exact nature of the infecting agent. The test shows the destruction of infecting power without any reference to the cause of the special virulence, which is demonstrated to be neutralized by certain chemical agents in a given amount. All of the above experiments made with the above-mentioned kinds of virus have been made upon the lower animals; but there is one kind of material which it is justifiable to use upon man himself, and with which numerous experiments of a very satisfactory character have been made. This material is vaccine virus. Fresh vaccine when inoculated into the arm of an unvaccinated person gives rise to a very characteristic result-the vaccine vescicle. The inference seems justified that any agent which will neutralize the specific infecting power of this material will also neutralize the small pox virus. Thus far it has not been definitely proved that the effective agent in vaccine virus is a living germ; but the numerous experiments made have shown that the chemical agents, which have the power of destroying the various kinds of infectious material heretofore mentioned, have also the power, in about the same amounts, of neutralizing vaccine virus, as shown by its failure to produce any results when inoculated into an unvaccinated person. In these experiments the more careful investigators have taken the precaution of vaccinating the same person with disinfected and non-disinfected virus from the same source. A successful vaccination with the non-disinfected virus shows that the individual is susceptible and the material good; failure to produce any result is evidence that the potency of the

disinfected virus has been destroyed by the chemical agent to which it was exposed.

(c). As already stated, it has been demonstrated that the infectious diseases of the lower animals, which have furnished the material for the experiments upon the disinfectants by the method of inoculation, are "germ diseases," and that the infectious agent is in each case a living micro-organism, belonging to the class known under the general name of Bacteria. The bacteria are vegetable organisms, which by reason of their minute size and simple organization, must be placed at the very foot of the scale of living things. But they make up in number and in rapidity of development for their minute size; and there is good reason for believing that the infectious diseases of man are also caused by pathogenic disease producing organism of the same class. Indeed, this has already been proved for some of these diseases and the evidence as regards several others is so convincing as to leave very little room for doubt.

Many of these disease germs are now known to us, not only by microscopic examination of the blood and tissues of infected ani. mals, but also by "culture experiments." That is, we are able to cultivate them artificially in a suitable media, and to study their mode of development, etc., in the labratory, quite independently of the animals from which our "pure cultures" were obtained in the first instance. The culture fluids used are prepared from the flesh of various animals; and when to one of these a certain quantity of gelatine is added, we have a "solid culture" medium, upon the surface of which some of these germs will grow most luxuriantly. To start such a "culture" it is only necessary to transfer, with proper precautions, a minute quantity of the infectious material to the surface of our culture medium, or into a fluid which has been found to be suitable for the growth of the particular organism which we desire to cultivate. A second culture is in the same way started from the first, and so on indefin. itely.

Now it is evident that these "pure cultures" furnish us a ready means for testing the power of various chemical agents to destroy the vitality of known disease germs, as shown by their failure to grow in a suitable culture medium after exposure for a given time to a given percentage of the disinfectant. Very many experiments of this nature have been made during the past three or four years. The reader who desires fuller details as to the method of conducting such experiments, and of the results obtained, is referred to the preliminary reports of the committee on disinfectants, of the American Public Health Association, published during the current year (1885) in the Medical News, Philadelphia, and which will, doubtless, also be published in full in the next annual volume of the association. We may say here, that the experimental data on record indicate that those agents which are efficient for the destruction of any one of the pathogenic organisms upon which experiments have been made, or of the harm

less species of the same class, e. g., the bacteria of putrefaction, are efficient for the destruction of all, in the absence of spores. There is, it is true, within certain limits, a difference in the resisting power of different organisms of this class to chemical agents. This is not, however, sufficiently marked to prevent the general statement that a disinfectant for one is a disinfectant for all, in the absence of spores.

The last clause of the above statement calls for an explanation, aud certain details with reference to the mode of reproduction of disease germs. All of the bacteria multiply by binary division; that is, one individual divides into two, and each member of the pair again into two, and so on. The spherical bacteria, known as micrococci, multiply only in this way, but the rod-shaped bacteria, or bacilli, also form spores. These spores correspond with the seeds of higher plants. They are highly refractive, oval or spherical bodies, which, under certain circumstances, make their appearance in the interior of the rods, which cease to multiply by binary division when spore formation has taken place. The point of special interest with reference to these spores is, that they have a resisting power to heat, and to the action of chemical disinfectants, far beyond that which is possessed by micrococci, or by bacilla without spores. The difference may be compared to the difference between a tender plant and its seeds to deleterous influences, such as extremes of heat and cold. Thus the spores of certain species of bacilla withstand a boiling temperature for several hours, while a temperature of 150 deg. Fahr. quickly kills most bacteria in the absence of spores. A similar difference is shown as regards the action of chemical agents. Certain agents, -e. g., sulphurous acid gas and carbolic acid,-which are extensively used as disinfectants, have been proved by exact experiments to be quite impotent for the destruction of spores. This being the case, it is advisable, in practical disinfection, always to use an agent which has the power of destroying spores, in those cases in which the exact nature of the disease germ has not been demonstrated. The cholera germ of Koch does not form spores; and there is good reason to believe that the same is true as regards the germs of yellow-fever, of scarlet fever, and of small pox, which have not yet been demonstrated. This inference is based upon evidence obtained in the practical use of disinfectants, and upon certain facts relating to the propogation of these diseases.

A second general statement, which is justified by the experimental evidence on record, is that agents which kill bacteria in a certain amount, prevent their multiplication in culture fluids, when present in quantities considerable less than are required to completely destroy vitality.

An agent, therefore, which, in a certain proportion and in a given time, acts as a "germicide" in a smaller quantity, may act as an antiseptic, i. e, may prevent putrefactive decomposition by restraining the development of the bacteria of putrefaction. Antiseptics also prevent or retard the development of pathogenic

bacteria. It follows from this that germicides are also antiseptics;. but the reverse of this proposition is not true as a general statement, for all antiseptics are not germicides. Thus alcohol,. common salt, sulphate of iron, and many other substances which are extensively used as antiseptics, have scarce any germicide power, even in concentrated solutions, and consequently would be entirely unreliable as disinfectants.

Practically, antiseptics may accomplish the some result in the long run as we obtain in a short time by the use of disinfectants. If, for example, we prevent the development of the germs of cholera, or of typhoid fever, in an infected privy vault, by the continued use of antiseptics these germs will in time lose their ability to grow, when introduced into a suitable culture medium. But in the meantime there is always the possibility that some of them may escape, with the fluid contents of the vault, into the surrounding soil, and contaminate some well or stream from which drinking water is obtained. For this reason privy vaults, cesspools and sewers should never be allowed to become infected. All infectious material, such as the dejections of patients with cholera or typhoid fever, should be destroyed at its source, in the sick-room; or, if it is ascertained that such material has been thrown into a privy vault, the entire contents of the vault should be promptly disinfected. The same rule applies to infectious material thrown upon the ground, or wherever it may be.

Finally, we desire to emphasize the following propositions: Disinfection consists in distinguishing the spark, killing the germ, which may light up an epidemic in the presence of a supply of cumbustible material-filth.

The object of general sanitary police is to remove this combustible material out of the way, so that no harm may result even if the spark be introduced.

Antiseptics and deodorants are useful when it is impracticable to remove offensive organic material from the vicinity of human habitations, but they are a poor substitute for cleanliness.

PART FIRST.

DISINFECTION.

It will be our aim in the present chapter to give reliable, practical directions with reference to the use of disifectants, and the best methods of disinfection. Keeping this object in view we shall recommend for disinfecting purposes only those agents named in the following list:

Group 1.

Disinfectants which have the power of destroying spores :
1. Fire.

2. Steam under pressure (25 lbs.).

3. Boiling water.

4. Chloride of lime (in solution).

5. Liquor soda chlorinatae.

6. Mercuric chloride (in solulion).

Group 2.

Disinfectants which are effective in the absence of spores:

7. Drv heat (230 deg. Fahr. for two hours).

8. Sulphur dioxide.

9. Carbolic acid.

10. Sulphate of copper (in solution.)

11. Chloride of zinc (in solution.)

Note. In the present state of knowledge, a division of disinfecting agents into two groups becomes necessary, unless we would entirely dispense with the use of those agents named in our second group, which cannot be relied upon for the destruction of spores and consequently cannot be recommended for the destruction of all kinds of infectious material. As this group includes several agents which are extensively used for disinfecting purposes, and which we believe to possess great practical value, we have considered it necessary to make this distinction. The present state of science, however, does not enable us to classify all infectious diseases in the same way, and in case of doubt it will always be advisable to use those agents included in group first. But in the absence of a precise knowledge of the nature of the germ, we may in certain cases be governed by the practical experience of sanitarians, and by experiments which have been made directly upon infectious material, e. g., on vaccine virus. In our recommendation we have taken account of this kind of evidence, as well as of labratorial experiments, in which known disease germs or harmless organisms of the same class have served as the test of disinfectant power.

We shall first give a brief account of the conditions of successful disinfection with these agents, as established by experimental data, and afterwards detail directions for their employment under the various circumstances in which disinfection is required.

1. Fire-It is hardly necessary that burning of infectious material, infected clothing, etc., is an effectual method of disposing of it. This method of disinfection is always to be recommended, when practicable or consistent with a due regard for economy and the rights of individuals. As a rule, articles of little value, which have been soiled with infectious material, had better be burned; and this is especially true of old clothing and bedding. But we have other efficient methods of disinfection, which make it unnecessary to sacrifice articles of value except under unusual circumstances.

2. Steam Under Pressure.-The disinfecting power of steam given off from boiling water in an open vessel does not differ from that of the water itself, but confined steam has a temperature cor